>
> No shared files such as databases should be using opportunistic locking.
>
> http://support.microsoft.com/default.aspx?scid=kb;en-us;Q129202
>
> Make a point of testing with at least two workstations as the norm.


What exactly did you mean with this point?

I suppose the registry value of "EnableOplocks"
on the server's
\HKEY_LOCAL_MACHINE\SYSTEM\CurrentControlSet
\Services\LanmanServer\Parameters


should be set to 0.

I tried to do that (with 0 and 1), but no difference in performances.
Actually, it is even worse with setting to 0. By default there was no registry key and I added it. If it was set to 1, performances were same as there was no key, but setting to 0 the access was slow even with first user.

It is interesting - when I connect to the server directly with 2 remote desktops at the same time, it isn't problem as it is with 2 users from real network workstations. So there must be something that could improve this multi-user access.